P733: Introduction of liquid-chromatography-mass spectrometry to evaluate protein purification in biochemistry lab
In the fast-paced world of modern science, exposure to multiple analytical techniques is increasingly important. Traditionally, techniques such as gel electrophoresis and determination of specific activity have been used to evaluate the effectiveness of a protein purification method. The use of liquid chromatography combined with electrospray mass spectrometry (LC-MS) to evaluate the purification of myoglobin from ground beef introduces concepts central to modern biochemical methods such as proteomics and peptide mapping. An autosampler was used for running the samples, but the students plotted their own data and discussed it in a lab report. LC-MS analysis was combined with gel electrophoretic analysis of their purified samples. This laboratory exercise was performed in a junior/senior level biochemistry course with 25 enrolled students.