P628: Research experiences in the undergraduate organic laboratory: Synthesis and studies of quinone methide precursors as acetylcholinesterase reactivators

Author: Christopher S. Callam, The Ohio State University, USA

Co-Author: Brent E. Sauner and Noel M. Paul, The Ohio State University, USA; Ryan J. Yoder, The Ohio State University, Marion Campus, USA

Date: 8/5/14

Time: 3:40 PM4:00 PM

Room: LMH 114

Related Symposium: S51

Acetylcholinesterase (AChE) is an essential enzyme in the human body, which hydrolyzes acetylcholine into choline for essential biochemical processes. Organophosphorus (OP) nerve-agents such as Sarin, Soman and Tabun are covalent inhibitors of AChE. Following exposure to OPs, AChE in inhibited and undergoes a subsequent irreversible aging process in which the OP-AChE adduct is dealkylated, resulting in the accumulation of excess acetylcholine in the central nervous system. Current oxime-based pharmaceuticals can only be used to treat the inhibited AChE and are ineffective on the aged AChE. One of our research modules for the undergraduate organic laboratories has a focus on developing these molecules for the reactivation of the aged AChE. Previous studies have shown that high energy quinone methides (QM) could potentially reverse the damage done to the active site on aged AChE through a kinetically favored alkylation of a phosphor-diester. Guided by computational methods, over 100 students have synthesized different QM precursors and investigated their ability to alkylate a model enzyme as a screen of initial reactivity. Students GC-MS, 1H NMR, 13C NMR and HPLC-TOF for characterization of their new compounds and activity screening efforts. At the conclusion of the study, a number of students expressed a desire to continue the project as undergraduate researchers. These experiments complement the classic synthetic experiments that comprise a large portion of the organic chemical laboratory experience and at the same time serve to ignite the interest in research problems at the introductory and advanced level.

P350: Innovations in Science methods courses: Preparing STEM educators through collaborative teaching

Author: Karen E. Irving, The Ohio State University, USA

Co-Author: Christopher S. Callam, The Ohio State University, USA

Date: 8/4/14

Time: 4:00 PM4:20 PM

Room: MAK A1161

Related Symposium: S32

At Ohio State University, a one-year Master of Education program supported by the Woodrow Wilson Foundation promoted collaborations between faculty from the Colleges of Education and Human Ecology (3), chemistry, physics, life science and geological sciences (Arts and Sciences, 5) and Engineering (2) to design and implement an innovative semester-long, 5-credit science methods course focused on inquiry and engineering design principles. The course prepares students for state licensure and increases their pedagogical content knowledge on topics identified by the Next Generation Science Standards (NGSS). Modular themes targeted five content areas in STEM: chemistry, earth science, life science, physics, and engineering. NGSS crosscutting concepts such as energy and matter, modeling, scientific and engineering design methods provided a common thread throughout the course. The presentation focuses on the innovations used in design of the chemistry module. The module was team-taught by a chemistry faculty member and an education faculty member. The module implemented a discussion format with an emphasis on chemical demonstrations and technology. Students designed lessons focused on a chemical demonstration to facilitate learning and understanding in the classroom. An emphasis was placed on the relationship between macro-scale, sub-microscale, and formulaic representations. Other activities included computer modeling, card sorts, concept maps, inquiry-based lab exercises, and building models. The course has been taught twice in the last two years with 21 total students completing the curriculum. The Woodrow Wilson Fellows commit to 3-years of teaching in a high-needs school on completion of the program.